method of direct smear - PMC

Letebrhan Weldemhret, Ataklti Hailu, Goyitom Gebremedhn, Hadish Bekuretsion, Gebreselassie Alemseged, Gebremicheal Gebreegziabher, Gebrhiwot Tesfahuney, Tesfaye Berhe, Lemlem Legesse, Kelali Kalayu, Mesfin Tesfay, Kibrti Mehari

PLoS One. 2020; 15(10): e0239342. Published online 2020 Oct 7. doi: 10.1371/journal.pone.0239342

PMCID:: PMC7540840

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To Test or Not? Xpert MTB/RIF as an Alternative to Smear Microscopy to Guide Line Probe Assay Testing for Drug-Resistant Tuberculosis

S. Pillay, M. de Vos, H. Sohn, Y. Ghebrekristos, T. Dolby, R. M. Warren, G. Theron

J Clin Microbiol. 2023 Jul; 61(7): e00017-23. Published online 2023 Jun 27. doi: 10.1128/jcm.00017-23

PMCID:: PMC10358166

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Sputum smear conversion and treatment outcomes among drug-resistant pulmonary tuberculosis patients in eastern Ethiopia: A 9-years data analysis

Mulugeta Gamachu, Alemayehu Deressa, Abdi Birhanu, Galana Mamo Ayana, Temam Beshir Raru, Belay Negash, Bedasa Taye Merga, Addisu Alemu, Fila Ahmed, Ahmed Mohammed, Ibsa M. Abdulahi, Lemma Demissei Regassa

Front Med (Lausanne) 2022; 9: 1007757. Published online 2022 Dec 1. doi: 10.3389/fmed.2022.1007757

PMCID:: PMC9751062

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Complete sputum smear monitoring among adults with pulmonary tuberculosis in central Uganda: evidence from a retrospective cohort study

Ronald Nsubuga, Norbert Adrawa, Stephen Okoboi, Alimah Komuhangi, Jonathan Izudi

BMC Infect Dis. 2022; 22: 191. Published online 2022 Feb 25. doi: 10.1186/s12879-022-07178-9

PMCID:: PMC8876256

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Prolongation of Acid-Fast Bacilli Sputum Smear Positivity in Patients with Multidrug-Resistant Pulmonary Tuberculosis

Sidwell Mvo, Carine Bokop, Benjamin Longo-Mbenza, Sandeep D. Vasaikar, Teke Apalata

Pathogens. 2023 Sep; 12(9): 1133. Published online 2023 Sep 5. doi: 10.3390/pathogens12091133

PMCID:: PMC10537881

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The role of the surface smear microbiome in the development of defective smear on surface-ripened red-smear cheese

Jasmine S. Ritschard, Lea Amato, Yadhu Kumar, Britta Müller, Leo Meile, Markus Schuppler

AIMS Microbiol. 2018; 4(4): 622–641. Published online 2018 Oct 25. doi: 10.3934/microbiol.2018.4.622

PMCID:: PMC6613336

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Non-actionable Results, Accuracy, and Effect of First- and Second-line Line Probe Assays for Diagnosing Drug-Resistant Tuberculosis, Including on Smear-Negative Specimens, in a High-Volume Laboratory

Samantha Pillay, Margaretha de Vos, Brigitta Derendinger, Elizabeth Maria Streicher, Tania Dolby, Leeré Ann Scott, Amy Debra Steinhobel, Rob Mark Warren, Grant Theron

Clin Infect Dis. 2023 Feb 1; 76(3): e920–e929. Published online 2022 Jul 5. doi: 10.1093/cid/ciac556

PMCID:: PMC7614164

Article PDF–700K

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Alcohol drinking delays the rate of sputum smear conversion among DR-TB patients in northwest Ethiopia; A retrospective follow-up study

Mehari Woldemariam Merid, Atalay Goshu Muluneh, Getahun Molla Kassa

PLoS One. 2022; 17(3): e0264852. Published online 2022 Mar 9. doi: 10.1371/journal.pone.0264852

PMCID:: PMC8906643

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Direct laser writing of 3D electrodes on flexible substrates

Morgan A. Brown, Kara M. Zappitelli, Loveprit Singh, Rachel C. Yuan, Melissa Bemrose, Valerie Brogden, David J. Miller, Matthew C. Smear, Stuart F. Cogan, Timothy J. Gardner

Nat Commun. 2023; 14: 3610. Published online 2023 Jun 17. doi: 10.1038/s41467-023-39152-7

PMCID:: PMC10276853

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Norfazilah Ahmad, Mazni Baharom, Azimatun Noor Aizuddin, Rohaya Ramli

PLoS One. 2021; 16(1): e0245304. Published online 2021 Jan 8. doi: 10.1371/journal.pone.0245304

PMCID:: PMC7793251

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Comparison of 3 diagnostic methods for pulmonary tuberculosis in suspected patients with negative sputum smear or no sputum

Xiaopeng Cheng, Lerong Chen, Wenli Wan, Jianping Peng, Liangliang Wu, Jing Xin, Jianying Cai

Medicine (Baltimore) 2024 Feb 9; 103(6): e37039. Published online 2024 Feb 9. doi: 10.1097/MD.0000000000037039

PMCID:: PMC10860950

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Frequency of Smear-Negative Tuberculosis in Northwest Iran

Mahya Pourostadi, M-Pill, Jalil Rashedi, MS, Behroz Mahdavi Poor, MS, Hossein Samadi Kafil, PhD, Maral Hariri-Akbari, MS, Mohammad Asgharzadeh, PhD

Iran J Med Sci. 2018 May; 43(3): 269–275.

PMCID:: PMC5993903

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Sputum smear non-conversion among adult persons with bacteriologically confirmed pulmonary tuberculosis in rural eastern Uganda

Jonathan Izudi, Imelda K. Tamwesigire, Francis Bajunirwe

J Clin Tuberc Other Mycobact Dis. 2020 Aug; 20: 100168. Published online 2020 May 30. doi: 10.1016/j.jctube.2020.100168

Correction in:: J Clin Tuberc Other Mycobact Dis. 2020 Dec; 21: 100177.

PMCID:: PMC7283142

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Risk factors for tuberculosis smear non-conversion in Eden district, Western Cape, South Africa, 2007–2013: a retrospective cohort study

Mandla Mlotshwa, Natasha Abraham, Moira Beery, Seymour Williams, Sandra Smit, Margot Uys, Carl Reddy, Andrew Medina-Marino

BMC Infect Dis. 2016; 16: 365. Published online 2016 Aug 2. doi: 10.1186/s12879-016-1712-y

PMCID:: PMC4971671

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Infectious and clinical tuberculosis trajectories: Bayesian modeling with case finding implications

Theresa S. Ryckman, David W. Dowdy, Emily A. Kendall

Proc Natl Acad Sci U S A. 2022 Dec 27; 119(52): e2211045119. Published online 2022 Dec 19. doi: 10.1073/pnas.2211045119

PMCID:: PMC9907102

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PMC Images search for method of direct smear

Figure 8

Detection of sputum samples with the new nano detection method.
Notes: Eight sputum samples were collected and detected with direct smear and the new sandwich method simultaneously. Positive results were only obtained for the six smear positive samples (S1–S6), including two one positive (1+) smear samples (S1, S2).

Detection of Mycobacterium tuberculosis based on H37Rv binding peptides using surface functionalized magnetic microspheres coupled with quantum dots – a nano detection method for Mycobacterium tuberculosis

Int J Nanomedicine. 2015;10:77-88.
FIG. 3.

N. asteroides filaments in a direct smear of sputum stained by the modified Kinyoun acid-fast method. Magnification, ×1,000.

Nocardiosis

J Clin Microbiol. 2003 Oct;41(10):4497-4501.
FIG. 1.

Physical appearance of smears. Smears were prepared from equal aliquots of a sputum sample processed by the CDC and USP smear methods. (i) A loopful from the purulent portion of sputum was taken in a 3-mm sterile wire loop and smeared onto a glass slide (direct). (ii) Two loopfuls of specimen processed by the CDC method were smeared onto a glass slide. (iii) Ten percent of the USP sputum was smeared onto a glass slide.

Utility of Universal Sample Processing Methodology, Combining Smear Microscopy, Culture, and PCR, for Diagnosis of Pulmonary Tuberculosis

J Clin Microbiol. 2005 Jun;43(6):2703-2708.
Figure 5

N. asteroides filaments in a direct smear of sputum stained by the modified Kinyoun acid-fast method. Magnification ×1,000 .

Nocardiosis in Kidney Disease Patients under Immunosuppressive Therapy: Case Report and Literature Review

Int J Med Sci. 2019;16(6):838-844.